CovidFranceV1, Main, Exploration, bibRecord, 000319

Ultra-High-Throughput Clinical Proteomics Reveals Classifiers of COVID-19 Infection.

Identifieur interne : 000319 ( Main/Exploration ); précédent : 000318; suivant : 000320

Ultra-High-Throughput Clinical Proteomics Reveals Classifiers of COVID-19 Infection.

Auteurs : Christoph B. Messner [Royaume-Uni] ; Vadim Demichev [Royaume-Uni] ; Daniel Wendisch [Allemagne] ; Laura Michalick [Allemagne] ; Matthew White [Royaume-Uni] ; Anja Freiwald [Allemagne] ; Kathrin Textoris-Taube [Allemagne] ; Spyros I. Vernardis [Royaume-Uni] ; Anna-Sophia Egger [Royaume-Uni] ; Marco Kreidl [Royaume-Uni] ; Daniela Ludwig [Allemagne] ; Christiane Kilian [Allemagne] ; Federica Agostini [Allemagne] ; Aleksej Zelezniak [Suède] ; Charlotte Thibeault [Allemagne] ; Moritz Pfeiffer [Allemagne] ; Stefan Hippenstiel [Allemagne] ; Andreas Hocke [Allemagne] ; Christof Von Kalle [Allemagne] ; Archie Campbell [Royaume-Uni] ; Caroline Hayward [Royaume-Uni] ; David J. Porteous [Royaume-Uni] ; Riccardo E. Marioni [Royaume-Uni] ; Claudia Langenberg [Royaume-Uni] ; Kathryn S. Lilley [Royaume-Uni] ; Wolfgang M. Kuebler [Allemagne] ; Michael Mülleder [Allemagne] ; Christian Drosten [Allemagne] ; Norbert Suttorp [Allemagne] ; Martin Witzenrath [Allemagne] ; Florian Kurth [Allemagne] ; Leif Erik Sander [Allemagne] ; Markus Ralser [Allemagne]

Source :

Cell systems [ 2405-4720 ] ; 2020.

RBID : pubmed:32619549

Descripteurs français

KwdFr :
- Adulte (MeSH), Adulte d'âge moyen (MeSH), Betacoronavirus (isolement et purification), Femelle (MeSH), Humains (MeSH), Infections à coronavirus (anatomopathologie), Infections à coronavirus (classification), Infections à coronavirus (sang), Infections à coronavirus (virologie), Jeune adulte (MeSH), Marqueurs biologiques (sang), Mâle (MeSH), Pandémies (classification), Pneumopathie virale (anatomopathologie), Pneumopathie virale (classification), Pneumopathie virale (sang), Pneumopathie virale (virologie), Protéines du sang (analyse), Protéines du sang (métabolisme), Protéomique (méthodes), Sujet âgé (MeSH), Sujet âgé de 80 ans ou plus (MeSH).
MESH :
- analyse : Protéines du sang.
- anatomopathologie : Infections à coronavirus, Pneumopathie virale.
- isolement et purification : Betacoronavirus.
- métabolisme : Protéines du sang.
- méthodes : Protéomique.
- sang : Infections à coronavirus, Marqueurs biologiques, Pandémies, Pneumopathie virale.
- virologie : Infections à coronavirus, Pneumopathie virale.
- Adulte, Adulte d'âge moyen, Femelle, Humains, Jeune adulte, Mâle, Sujet âgé, Sujet âgé de 80 ans ou plus.

English descriptors

KwdEn :
- Adult (MeSH), Aged (MeSH), Aged, 80 and over (MeSH), Betacoronavirus (isolation & purification), Biomarkers (blood), Blood Proteins (analysis), Blood Proteins (metabolism), Coronavirus Infections (blood), Coronavirus Infections (classification), Coronavirus Infections (pathology), Coronavirus Infections (virology), Female (MeSH), Humans (MeSH), Male (MeSH), Middle Aged (MeSH), Pandemics (classification), Pneumonia, Viral (blood), Pneumonia, Viral (classification), Pneumonia, Viral (pathology), Pneumonia, Viral (virology), Proteomics (methods), Young Adult (MeSH).
MESH :
- chemical , analysis : Blood Proteins.
- chemical , blood : Biomarkers.
- blood : Coronavirus Infections, Pneumonia, Viral.
- classification : Coronavirus Infections, Pandemics, Pneumonia, Viral.
- isolation & purification : Betacoronavirus.
- chemical , metabolism : Blood Proteins.
- methods : Proteomics.
- pathology : Coronavirus Infections, Pneumonia, Viral.
- virology : Coronavirus Infections, Pneumonia, Viral.
- Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Young Adult.

Abstract

The COVID-19 pandemic is an unprecedented global challenge, and point-of-care diagnostic classifiers are urgently required. Here, we present a platform for ultra-high-throughput serum and plasma proteomics that builds on ISO13485 standardization to facilitate simple implementation in regulated clinical laboratories. Our low-cost workflow handles up to 180 samples per day, enables high precision quantification, and reduces batch effects for large-scale and longitudinal studies. We use our platform on samples collected from a cohort of early hospitalized cases of the SARS-CoV-2 pandemic and identify 27 potential biomarkers that are differentially expressed depending on the WHO severity grade of COVID-19. They include complement factors, the coagulation system, inflammation modulators, and pro-inflammatory factors upstream and downstream of interleukin 6. All protocols and software for implementing our approach are freely available. In total, this work supports the development of routine proteomic assays to aid clinical decision making and generate hypotheses about potential COVID-19 therapeutic targets.

DOI: 10.1016/j.cels.2020.05.012
PubMed: 32619549
PubMed Central: PMC7264033

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000B13
to stream Main, to step Curation: 000B13

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ultra-High-Throughput Clinical Proteomics Reveals Classifiers of COVID-19 Infection.</title>
<author><name sortKey="Messner, Christoph B" sort="Messner, Christoph B" uniqKey="Messner C" first="Christoph B" last="Messner">Christoph B. Messner</name>
<affiliation wicri:level="1"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT</wicri:regionArea>
<wicri:noRegion>London NW11AT</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Demichev, Vadim" sort="Demichev, Vadim" uniqKey="Demichev V" first="Vadim" last="Demichev">Vadim Demichev</name>
<affiliation wicri:level="4"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Department of Biochemistry, The University of Cambridge, Cambridge CB21GA, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Department of Biochemistry, The University of Cambridge, Cambridge CB21GA</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wendisch, Daniel" sort="Wendisch, Daniel" uniqKey="Wendisch D" first="Daniel" last="Wendisch">Daniel Wendisch</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Michalick, Laura" sort="Michalick, Laura" uniqKey="Michalick L" first="Laura" last="Michalick">Laura Michalick</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Institute of Physiology, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Institute of Physiology, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="White, Matthew" sort="White, Matthew" uniqKey="White M" first="Matthew" last="White">Matthew White</name>
<affiliation wicri:level="1"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT</wicri:regionArea>
<wicri:noRegion>London NW11AT</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Freiwald, Anja" sort="Freiwald, Anja" uniqKey="Freiwald A" first="Anja" last="Freiwald">Anja Freiwald</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin, Germany; Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin, Germany; Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Textoris Taube, Kathrin" sort="Textoris Taube, Kathrin" uniqKey="Textoris Taube K" first="Kathrin" last="Textoris-Taube">Kathrin Textoris-Taube</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Vernardis, Spyros I" sort="Vernardis, Spyros I" uniqKey="Vernardis S" first="Spyros I" last="Vernardis">Spyros I. Vernardis</name>
<affiliation wicri:level="1"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT</wicri:regionArea>
<wicri:noRegion>London NW11AT</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Egger, Anna Sophia" sort="Egger, Anna Sophia" uniqKey="Egger A" first="Anna-Sophia" last="Egger">Anna-Sophia Egger</name>
<affiliation wicri:level="1"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT</wicri:regionArea>
<wicri:noRegion>London NW11AT</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kreidl, Marco" sort="Kreidl, Marco" uniqKey="Kreidl M" first="Marco" last="Kreidl">Marco Kreidl</name>
<affiliation wicri:level="1"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT</wicri:regionArea>
<wicri:noRegion>London NW11AT</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ludwig, Daniela" sort="Ludwig, Daniela" uniqKey="Ludwig D" first="Daniela" last="Ludwig">Daniela Ludwig</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kilian, Christiane" sort="Kilian, Christiane" uniqKey="Kilian C" first="Christiane" last="Kilian">Christiane Kilian</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Agostini, Federica" sort="Agostini, Federica" uniqKey="Agostini F" first="Federica" last="Agostini">Federica Agostini</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zelezniak, Aleksej" sort="Zelezniak, Aleksej" uniqKey="Zelezniak A" first="Aleksej" last="Zelezniak">Aleksej Zelezniak</name>
<affiliation wicri:level="1"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg 412 96, Sweden.</nlm:affiliation>
<country xml:lang="fr">Suède</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg 412 96</wicri:regionArea>
<wicri:noRegion>Gothenburg 412 96</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Thibeault, Charlotte" sort="Thibeault, Charlotte" uniqKey="Thibeault C" first="Charlotte" last="Thibeault">Charlotte Thibeault</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pfeiffer, Moritz" sort="Pfeiffer, Moritz" uniqKey="Pfeiffer M" first="Moritz" last="Pfeiffer">Moritz Pfeiffer</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hippenstiel, Stefan" sort="Hippenstiel, Stefan" uniqKey="Hippenstiel S" first="Stefan" last="Hippenstiel">Stefan Hippenstiel</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hocke, Andreas" sort="Hocke, Andreas" uniqKey="Hocke A" first="Andreas" last="Hocke">Andreas Hocke</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Von Kalle, Christof" sort="Von Kalle, Christof" uniqKey="Von Kalle C" first="Christof" last="Von Kalle">Christof Von Kalle</name>
<affiliation wicri:level="3"><nlm:affiliation>Berlin Institute of Health (BIH) and Charité Universitätsmedizin, Clinical Study Center (CSC), 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Berlin Institute of Health (BIH) and Charité Universitätsmedizin, Clinical Study Center (CSC), 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Campbell, Archie" sort="Campbell, Archie" uniqKey="Campbell A" first="Archie" last="Campbell">Archie Campbell</name>
<affiliation wicri:level="4"><nlm:affiliation>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK; Usher Institute, University of Edinburgh, Nine, Edinburgh Bioquarter, 9 Little France Road, Edinburgh EH16 4UX, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK; Usher Institute, University of Edinburgh, Nine, Edinburgh Bioquarter, 9 Little France Road, Edinburgh EH16 4UX</wicri:regionArea>
<orgName type="university">Université d'Édimbourg</orgName>
<placeName><settlement type="city">Édimbourg</settlement>
<region type="country">Écosse</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hayward, Caroline" sort="Hayward, Caroline" uniqKey="Hayward C" first="Caroline" last="Hayward">Caroline Hayward</name>
<affiliation wicri:level="4"><nlm:affiliation>MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU</wicri:regionArea>
<orgName type="university">Université d'Édimbourg</orgName>
<placeName><settlement type="city">Édimbourg</settlement>
<region type="country">Écosse</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Porteous, David J" sort="Porteous, David J" uniqKey="Porteous D" first="David J" last="Porteous">David J. Porteous</name>
<affiliation wicri:level="4"><nlm:affiliation>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU</wicri:regionArea>
<orgName type="university">Université d'Édimbourg</orgName>
<placeName><settlement type="city">Édimbourg</settlement>
<region type="country">Écosse</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Marioni, Riccardo E" sort="Marioni, Riccardo E" uniqKey="Marioni R" first="Riccardo E" last="Marioni">Riccardo E. Marioni</name>
<affiliation wicri:level="4"><nlm:affiliation>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU</wicri:regionArea>
<orgName type="university">Université d'Édimbourg</orgName>
<placeName><settlement type="city">Édimbourg</settlement>
<region type="country">Écosse</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Langenberg, Claudia" sort="Langenberg, Claudia" uniqKey="Langenberg C" first="Claudia" last="Langenberg">Claudia Langenberg</name>
<affiliation wicri:level="4"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; MRC Epidemiology Unit, Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; MRC Epidemiology Unit, Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lilley, Kathryn S" sort="Lilley, Kathryn S" uniqKey="Lilley K" first="Kathryn S" last="Lilley">Kathryn S. Lilley</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry, The University of Cambridge, Cambridge CB21GA, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Biochemistry, The University of Cambridge, Cambridge CB21GA</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kuebler, Wolfgang M" sort="Kuebler, Wolfgang M" uniqKey="Kuebler W" first="Wolfgang M" last="Kuebler">Wolfgang M. Kuebler</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Institute of Physiology, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Institute of Physiology, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mulleder, Michael" sort="Mulleder, Michael" uniqKey="Mulleder M" first="Michael" last="Mülleder">Michael Mülleder</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Department of Virology, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Department of Virology, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Suttorp, Norbert" sort="Suttorp, Norbert" uniqKey="Suttorp N" first="Norbert" last="Suttorp">Norbert Suttorp</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Witzenrath, Martin" sort="Witzenrath, Martin" uniqKey="Witzenrath M" first="Martin" last="Witzenrath">Martin Witzenrath</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kurth, Florian" sort="Kurth, Florian" uniqKey="Kurth F" first="Florian" last="Kurth">Florian Kurth</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany; Department of Tropical Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany; Department of Tropical Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg</wicri:regionArea>
<placeName><settlement type="city">Hambourg</settlement>
<region type="land" nuts="2">Hambourg</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sander, Leif Erik" sort="Sander, Leif Erik" uniqKey="Sander L" first="Leif Erik" last="Sander">Leif Erik Sander</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ralser, Markus" sort="Ralser, Markus" uniqKey="Ralser M" first="Markus" last="Ralser">Markus Ralser</name>
<affiliation wicri:level="3"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany. Electronic address: markus.ralser@charite.de.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2020">2020</date>
<idno type="RBID">pubmed:32619549</idno>
<idno type="pmid">32619549</idno>
<idno type="doi">10.1016/j.cels.2020.05.012</idno>
<idno type="pmc">PMC7264033</idno>
<idno type="wicri:Area/Main/Corpus">000B13</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000B13</idno>
<idno type="wicri:Area/Main/Curation">000B13</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000B13</idno>
<idno type="wicri:Area/Main/Exploration">000B13</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Ultra-High-Throughput Clinical Proteomics Reveals Classifiers of COVID-19 Infection.</title>
<author><name sortKey="Messner, Christoph B" sort="Messner, Christoph B" uniqKey="Messner C" first="Christoph B" last="Messner">Christoph B. Messner</name>
<affiliation wicri:level="1"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT</wicri:regionArea>
<wicri:noRegion>London NW11AT</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Demichev, Vadim" sort="Demichev, Vadim" uniqKey="Demichev V" first="Vadim" last="Demichev">Vadim Demichev</name>
<affiliation wicri:level="4"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Department of Biochemistry, The University of Cambridge, Cambridge CB21GA, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Department of Biochemistry, The University of Cambridge, Cambridge CB21GA</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wendisch, Daniel" sort="Wendisch, Daniel" uniqKey="Wendisch D" first="Daniel" last="Wendisch">Daniel Wendisch</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Michalick, Laura" sort="Michalick, Laura" uniqKey="Michalick L" first="Laura" last="Michalick">Laura Michalick</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Institute of Physiology, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Institute of Physiology, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="White, Matthew" sort="White, Matthew" uniqKey="White M" first="Matthew" last="White">Matthew White</name>
<affiliation wicri:level="1"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT</wicri:regionArea>
<wicri:noRegion>London NW11AT</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Freiwald, Anja" sort="Freiwald, Anja" uniqKey="Freiwald A" first="Anja" last="Freiwald">Anja Freiwald</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin, Germany; Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin, Germany; Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Textoris Taube, Kathrin" sort="Textoris Taube, Kathrin" uniqKey="Textoris Taube K" first="Kathrin" last="Textoris-Taube">Kathrin Textoris-Taube</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Vernardis, Spyros I" sort="Vernardis, Spyros I" uniqKey="Vernardis S" first="Spyros I" last="Vernardis">Spyros I. Vernardis</name>
<affiliation wicri:level="1"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT</wicri:regionArea>
<wicri:noRegion>London NW11AT</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Egger, Anna Sophia" sort="Egger, Anna Sophia" uniqKey="Egger A" first="Anna-Sophia" last="Egger">Anna-Sophia Egger</name>
<affiliation wicri:level="1"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT</wicri:regionArea>
<wicri:noRegion>London NW11AT</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kreidl, Marco" sort="Kreidl, Marco" uniqKey="Kreidl M" first="Marco" last="Kreidl">Marco Kreidl</name>
<affiliation wicri:level="1"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT</wicri:regionArea>
<wicri:noRegion>London NW11AT</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ludwig, Daniela" sort="Ludwig, Daniela" uniqKey="Ludwig D" first="Daniela" last="Ludwig">Daniela Ludwig</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kilian, Christiane" sort="Kilian, Christiane" uniqKey="Kilian C" first="Christiane" last="Kilian">Christiane Kilian</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Agostini, Federica" sort="Agostini, Federica" uniqKey="Agostini F" first="Federica" last="Agostini">Federica Agostini</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zelezniak, Aleksej" sort="Zelezniak, Aleksej" uniqKey="Zelezniak A" first="Aleksej" last="Zelezniak">Aleksej Zelezniak</name>
<affiliation wicri:level="1"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg 412 96, Sweden.</nlm:affiliation>
<country xml:lang="fr">Suède</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg 412 96</wicri:regionArea>
<wicri:noRegion>Gothenburg 412 96</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Thibeault, Charlotte" sort="Thibeault, Charlotte" uniqKey="Thibeault C" first="Charlotte" last="Thibeault">Charlotte Thibeault</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pfeiffer, Moritz" sort="Pfeiffer, Moritz" uniqKey="Pfeiffer M" first="Moritz" last="Pfeiffer">Moritz Pfeiffer</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hippenstiel, Stefan" sort="Hippenstiel, Stefan" uniqKey="Hippenstiel S" first="Stefan" last="Hippenstiel">Stefan Hippenstiel</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hocke, Andreas" sort="Hocke, Andreas" uniqKey="Hocke A" first="Andreas" last="Hocke">Andreas Hocke</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Von Kalle, Christof" sort="Von Kalle, Christof" uniqKey="Von Kalle C" first="Christof" last="Von Kalle">Christof Von Kalle</name>
<affiliation wicri:level="3"><nlm:affiliation>Berlin Institute of Health (BIH) and Charité Universitätsmedizin, Clinical Study Center (CSC), 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Berlin Institute of Health (BIH) and Charité Universitätsmedizin, Clinical Study Center (CSC), 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Campbell, Archie" sort="Campbell, Archie" uniqKey="Campbell A" first="Archie" last="Campbell">Archie Campbell</name>
<affiliation wicri:level="4"><nlm:affiliation>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK; Usher Institute, University of Edinburgh, Nine, Edinburgh Bioquarter, 9 Little France Road, Edinburgh EH16 4UX, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK; Usher Institute, University of Edinburgh, Nine, Edinburgh Bioquarter, 9 Little France Road, Edinburgh EH16 4UX</wicri:regionArea>
<orgName type="university">Université d'Édimbourg</orgName>
<placeName><settlement type="city">Édimbourg</settlement>
<region type="country">Écosse</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hayward, Caroline" sort="Hayward, Caroline" uniqKey="Hayward C" first="Caroline" last="Hayward">Caroline Hayward</name>
<affiliation wicri:level="4"><nlm:affiliation>MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU</wicri:regionArea>
<orgName type="university">Université d'Édimbourg</orgName>
<placeName><settlement type="city">Édimbourg</settlement>
<region type="country">Écosse</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Porteous, David J" sort="Porteous, David J" uniqKey="Porteous D" first="David J" last="Porteous">David J. Porteous</name>
<affiliation wicri:level="4"><nlm:affiliation>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU</wicri:regionArea>
<orgName type="university">Université d'Édimbourg</orgName>
<placeName><settlement type="city">Édimbourg</settlement>
<region type="country">Écosse</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Marioni, Riccardo E" sort="Marioni, Riccardo E" uniqKey="Marioni R" first="Riccardo E" last="Marioni">Riccardo E. Marioni</name>
<affiliation wicri:level="4"><nlm:affiliation>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU</wicri:regionArea>
<orgName type="university">Université d'Édimbourg</orgName>
<placeName><settlement type="city">Édimbourg</settlement>
<region type="country">Écosse</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Langenberg, Claudia" sort="Langenberg, Claudia" uniqKey="Langenberg C" first="Claudia" last="Langenberg">Claudia Langenberg</name>
<affiliation wicri:level="4"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; MRC Epidemiology Unit, Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; MRC Epidemiology Unit, Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lilley, Kathryn S" sort="Lilley, Kathryn S" uniqKey="Lilley K" first="Kathryn S" last="Lilley">Kathryn S. Lilley</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry, The University of Cambridge, Cambridge CB21GA, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Department of Biochemistry, The University of Cambridge, Cambridge CB21GA</wicri:regionArea>
<orgName type="university">Université de Cambridge</orgName>
<placeName><settlement type="city">Cambridge</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Angleterre de l'Est</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kuebler, Wolfgang M" sort="Kuebler, Wolfgang M" uniqKey="Kuebler W" first="Wolfgang M" last="Kuebler">Wolfgang M. Kuebler</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Institute of Physiology, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Institute of Physiology, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mulleder, Michael" sort="Mulleder, Michael" uniqKey="Mulleder M" first="Michael" last="Mülleder">Michael Mülleder</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Department of Virology, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Department of Virology, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Suttorp, Norbert" sort="Suttorp, Norbert" uniqKey="Suttorp N" first="Norbert" last="Suttorp">Norbert Suttorp</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Witzenrath, Martin" sort="Witzenrath, Martin" uniqKey="Witzenrath M" first="Martin" last="Witzenrath">Martin Witzenrath</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kurth, Florian" sort="Kurth, Florian" uniqKey="Kurth F" first="Florian" last="Kurth">Florian Kurth</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany; Department of Tropical Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany; Department of Tropical Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg</wicri:regionArea>
<placeName><settlement type="city">Hambourg</settlement>
<region type="land" nuts="2">Hambourg</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sander, Leif Erik" sort="Sander, Leif Erik" uniqKey="Sander L" first="Leif Erik" last="Sander">Leif Erik Sander</name>
<affiliation wicri:level="3"><nlm:affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ralser, Markus" sort="Ralser, Markus" uniqKey="Ralser M" first="Markus" last="Ralser">Markus Ralser</name>
<affiliation wicri:level="3"><nlm:affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany. Electronic address: markus.ralser@charite.de.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Cell systems</title>
<idno type="eISSN">2405-4720</idno>
<imprint><date when="2020" type="published">2020</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult (MeSH)</term>
<term>Aged (MeSH)</term>
<term>Aged, 80 and over (MeSH)</term>
<term>Betacoronavirus (isolation & purification)</term>
<term>Biomarkers (blood)</term>
<term>Blood Proteins (analysis)</term>
<term>Blood Proteins (metabolism)</term>
<term>Coronavirus Infections (blood)</term>
<term>Coronavirus Infections (classification)</term>
<term>Coronavirus Infections (pathology)</term>
<term>Coronavirus Infections (virology)</term>
<term>Female (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Male (MeSH)</term>
<term>Middle Aged (MeSH)</term>
<term>Pandemics (classification)</term>
<term>Pneumonia, Viral (blood)</term>
<term>Pneumonia, Viral (classification)</term>
<term>Pneumonia, Viral (pathology)</term>
<term>Pneumonia, Viral (virology)</term>
<term>Proteomics (methods)</term>
<term>Young Adult (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adulte (MeSH)</term>
<term>Adulte d'âge moyen (MeSH)</term>
<term>Betacoronavirus (isolement et purification)</term>
<term>Femelle (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Infections à coronavirus (anatomopathologie)</term>
<term>Infections à coronavirus (classification)</term>
<term>Infections à coronavirus (sang)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Jeune adulte (MeSH)</term>
<term>Marqueurs biologiques (sang)</term>
<term>Mâle (MeSH)</term>
<term>Pandémies (classification)</term>
<term>Pneumopathie virale (anatomopathologie)</term>
<term>Pneumopathie virale (classification)</term>
<term>Pneumopathie virale (sang)</term>
<term>Pneumopathie virale (virologie)</term>
<term>Protéines du sang (analyse)</term>
<term>Protéines du sang (métabolisme)</term>
<term>Protéomique (méthodes)</term>
<term>Sujet âgé (MeSH)</term>
<term>Sujet âgé de 80 ans ou plus (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Blood Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Biomarkers</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines du sang</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Infections à coronavirus</term>
<term>Pneumopathie virale</term>
</keywords>
<keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Coronavirus Infections</term>
<term>Pneumonia, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronavirus Infections</term>
<term>Pandemics</term>
<term>Pneumonia, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Betacoronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Betacoronavirus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Blood Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines du sang</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Protéomique</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Pneumonia, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Infections à coronavirus</term>
<term>Marqueurs biologiques</term>
<term>Pandémies</term>
<term>Pneumopathie virale</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term>
<term>Pneumopathie virale</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Pneumonia, Viral</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Adult</term>
<term>Aged</term>
<term>Aged, 80 and over</term>
<term>Female</term>
<term>Humans</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Young Adult</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Femelle</term>
<term>Humains</term>
<term>Jeune adulte</term>
<term>Mâle</term>
<term>Sujet âgé</term>
<term>Sujet âgé de 80 ans ou plus</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The COVID-19 pandemic is an unprecedented global challenge, and point-of-care diagnostic classifiers are urgently required. Here, we present a platform for ultra-high-throughput serum and plasma proteomics that builds on ISO13485 standardization to facilitate simple implementation in regulated clinical laboratories. Our low-cost workflow handles up to 180 samples per day, enables high precision quantification, and reduces batch effects for large-scale and longitudinal studies. We use our platform on samples collected from a cohort of early hospitalized cases of the SARS-CoV-2 pandemic and identify 27 potential biomarkers that are differentially expressed depending on the WHO severity grade of COVID-19. They include complement factors, the coagulation system, inflammation modulators, and pro-inflammatory factors upstream and downstream of interleukin 6. All protocols and software for implementing our approach are freely available. In total, this work supports the development of routine proteomic assays to aid clinical decision making and generate hypotheses about potential COVID-19 therapeutic targets.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32619549</PMID>
<DateCompleted><Year>2020</Year>
<Month>08</Month>
<Day>13</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>09</Month>
<Day>20</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2405-4720</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>11</Volume>
<Issue>1</Issue>
<PubDate><Year>2020</Year>
<Month>07</Month>
<Day>22</Day>
</PubDate>
</JournalIssue>
<Title>Cell systems</Title>
<ISOAbbreviation>Cell Syst</ISOAbbreviation>
</Journal>
<ArticleTitle>Ultra-High-Throughput Clinical Proteomics Reveals Classifiers of COVID-19 Infection.</ArticleTitle>
<Pagination><MedlinePgn>11-24.e4</MedlinePgn>
</Pagination>
<ELocationID EIdType="pii" ValidYN="Y">S2405-4712(20)30197-6</ELocationID>
<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cels.2020.05.012</ELocationID>
<Abstract><AbstractText>The COVID-19 pandemic is an unprecedented global challenge, and point-of-care diagnostic classifiers are urgently required. Here, we present a platform for ultra-high-throughput serum and plasma proteomics that builds on ISO13485 standardization to facilitate simple implementation in regulated clinical laboratories. Our low-cost workflow handles up to 180 samples per day, enables high precision quantification, and reduces batch effects for large-scale and longitudinal studies. We use our platform on samples collected from a cohort of early hospitalized cases of the SARS-CoV-2 pandemic and identify 27 potential biomarkers that are differentially expressed depending on the WHO severity grade of COVID-19. They include complement factors, the coagulation system, inflammation modulators, and pro-inflammatory factors upstream and downstream of interleukin 6. All protocols and software for implementing our approach are freely available. In total, this work supports the development of routine proteomic assays to aid clinical decision making and generate hypotheses about potential COVID-19 therapeutic targets.</AbstractText>
<CopyrightInformation>Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Messner</LastName>
<ForeName>Christoph B</ForeName>
<Initials>CB</Initials>
<AffiliationInfo><Affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Demichev</LastName>
<ForeName>Vadim</ForeName>
<Initials>V</Initials>
<AffiliationInfo><Affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Department of Biochemistry, The University of Cambridge, Cambridge CB21GA, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wendisch</LastName>
<ForeName>Daniel</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Michalick</LastName>
<ForeName>Laura</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Institute of Physiology, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>White</LastName>
<ForeName>Matthew</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Freiwald</LastName>
<ForeName>Anja</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin, Germany; Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Textoris-Taube</LastName>
<ForeName>Kathrin</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Vernardis</LastName>
<ForeName>Spyros I</ForeName>
<Initials>SI</Initials>
<AffiliationInfo><Affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Egger</LastName>
<ForeName>Anna-Sophia</ForeName>
<Initials>AS</Initials>
<AffiliationInfo><Affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kreidl</LastName>
<ForeName>Marco</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ludwig</LastName>
<ForeName>Daniela</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kilian</LastName>
<ForeName>Christiane</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Agostini</LastName>
<ForeName>Federica</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zelezniak</LastName>
<ForeName>Aleksej</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg 412 96, Sweden.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Thibeault</LastName>
<ForeName>Charlotte</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Pfeiffer</LastName>
<ForeName>Moritz</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hippenstiel</LastName>
<ForeName>Stefan</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hocke</LastName>
<ForeName>Andreas</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>von Kalle</LastName>
<ForeName>Christof</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Berlin Institute of Health (BIH) and Charité Universitätsmedizin, Clinical Study Center (CSC), 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Campbell</LastName>
<ForeName>Archie</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK; Usher Institute, University of Edinburgh, Nine, Edinburgh Bioquarter, 9 Little France Road, Edinburgh EH16 4UX, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hayward</LastName>
<ForeName>Caroline</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Porteous</LastName>
<ForeName>David J</ForeName>
<Initials>DJ</Initials>
<AffiliationInfo><Affiliation>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Marioni</LastName>
<ForeName>Riccardo E</ForeName>
<Initials>RE</Initials>
<AffiliationInfo><Affiliation>Centre for Genomic and Experimental Medicine, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Langenberg</LastName>
<ForeName>Claudia</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; MRC Epidemiology Unit, Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lilley</LastName>
<ForeName>Kathryn S</ForeName>
<Initials>KS</Initials>
<AffiliationInfo><Affiliation>Department of Biochemistry, The University of Cambridge, Cambridge CB21GA, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kuebler</LastName>
<ForeName>Wolfgang M</ForeName>
<Initials>WM</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Institute of Physiology, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mülleder</LastName>
<ForeName>Michael</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Core Facility - High-Throughput Mass Spectrometry, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Drosten</LastName>
<ForeName>Christian</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Department of Virology, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Suttorp</LastName>
<ForeName>Norbert</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Witzenrath</LastName>
<ForeName>Martin</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kurth</LastName>
<ForeName>Florian</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany; Department of Tropical Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sander</LastName>
<ForeName>Leif Erik</ForeName>
<Initials>LE</Initials>
<AffiliationInfo><Affiliation>Charité Universitätsmedizin, Berlin, Department of Infectious Diseases and Respiratory Medicine, 10117 Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ralser</LastName>
<ForeName>Markus</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London NW11AT, UK; Charité Universitätsmedizin, Department of Biochemistry, 10117 Berlin, Germany. Electronic address: markus.ralser@charite.de.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><Acronym>WT_</Acronym>
<Agency>Wellcome Trust</Agency>
<Country>United Kingdom</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2020</Year>
<Month>06</Month>
<Day>02</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Cell Syst</MedlineTA>
<NlmUniqueID>101656080</NlmUniqueID>
<ISSNLinking>2405-4712</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D015415">Biomarkers</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D001798">Blood Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<SupplMeshList><SupplMeshName Type="Disease" UI="C000657245">COVID-19</SupplMeshName>
<SupplMeshName Type="Organism" UI="C000656484">severe acute respiratory syndrome coronavirus 2</SupplMeshName>
</SupplMeshList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000369" MajorTopicYN="N">Aged, 80 and over</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000073640" MajorTopicYN="N">Betacoronavirus</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015415" MajorTopicYN="N">Biomarkers</DescriptorName>
<QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001798" MajorTopicYN="N">Blood Proteins</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName>
<QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName>
<QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName>
<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D058873" MajorTopicYN="N">Pandemics</DescriptorName>
<QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011024" MajorTopicYN="N">Pneumonia, Viral</DescriptorName>
<QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName>
<QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName>
<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D055815" MajorTopicYN="N">Young Adult</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">COVID-19 infection</Keyword>
<Keyword MajorTopicYN="Y">SWATH-MS</Keyword>
<Keyword MajorTopicYN="Y">antiviral immune response</Keyword>
<Keyword MajorTopicYN="Y">clinical classifiers</Keyword>
<Keyword MajorTopicYN="Y">high-throughput proteomics</Keyword>
<Keyword MajorTopicYN="Y">mass spectrometry</Keyword>
</KeywordList>
<CoiStatement>Declaration of Interests The authors declare no competing interests.</CoiStatement>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year>
<Month>04</Month>
<Day>26</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2020</Year>
<Month>05</Month>
<Day>22</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2020</Year>
<Month>05</Month>
<Day>27</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2020</Year>
<Month>7</Month>
<Day>4</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>8</Month>
<Day>14</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2020</Year>
<Month>7</Month>
<Day>4</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">32619549</ArticleId>
<ArticleId IdType="pii">S2405-4712(20)30197-6</ArticleId>
<ArticleId IdType="doi">10.1016/j.cels.2020.05.012</ArticleId>
<ArticleId IdType="pmc">PMC7264033</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Allemagne</li>
<li>Royaume-Uni</li>
<li>Suède</li>
</country>
<region><li>Angleterre</li>
<li>Angleterre de l'Est</li>
<li>Berlin</li>
<li>Hambourg</li>
<li>Écosse</li>
</region>
<settlement><li>Berlin</li>
<li>Cambridge</li>
<li>Hambourg</li>
<li>Édimbourg</li>
</settlement>
<orgName><li>Université d'Édimbourg</li>
<li>Université de Cambridge</li>
</orgName>
</list>
<tree><country name="Royaume-Uni"><noRegion><name sortKey="Messner, Christoph B" sort="Messner, Christoph B" uniqKey="Messner C" first="Christoph B" last="Messner">Christoph B. Messner</name>
</noRegion>
<name sortKey="Campbell, Archie" sort="Campbell, Archie" uniqKey="Campbell A" first="Archie" last="Campbell">Archie Campbell</name>
<name sortKey="Demichev, Vadim" sort="Demichev, Vadim" uniqKey="Demichev V" first="Vadim" last="Demichev">Vadim Demichev</name>
<name sortKey="Egger, Anna Sophia" sort="Egger, Anna Sophia" uniqKey="Egger A" first="Anna-Sophia" last="Egger">Anna-Sophia Egger</name>
<name sortKey="Hayward, Caroline" sort="Hayward, Caroline" uniqKey="Hayward C" first="Caroline" last="Hayward">Caroline Hayward</name>
<name sortKey="Kreidl, Marco" sort="Kreidl, Marco" uniqKey="Kreidl M" first="Marco" last="Kreidl">Marco Kreidl</name>
<name sortKey="Langenberg, Claudia" sort="Langenberg, Claudia" uniqKey="Langenberg C" first="Claudia" last="Langenberg">Claudia Langenberg</name>
<name sortKey="Lilley, Kathryn S" sort="Lilley, Kathryn S" uniqKey="Lilley K" first="Kathryn S" last="Lilley">Kathryn S. Lilley</name>
<name sortKey="Marioni, Riccardo E" sort="Marioni, Riccardo E" uniqKey="Marioni R" first="Riccardo E" last="Marioni">Riccardo E. Marioni</name>
<name sortKey="Porteous, David J" sort="Porteous, David J" uniqKey="Porteous D" first="David J" last="Porteous">David J. Porteous</name>
<name sortKey="Vernardis, Spyros I" sort="Vernardis, Spyros I" uniqKey="Vernardis S" first="Spyros I" last="Vernardis">Spyros I. Vernardis</name>
<name sortKey="White, Matthew" sort="White, Matthew" uniqKey="White M" first="Matthew" last="White">Matthew White</name>
</country>
<country name="Allemagne"><region name="Berlin"><name sortKey="Wendisch, Daniel" sort="Wendisch, Daniel" uniqKey="Wendisch D" first="Daniel" last="Wendisch">Daniel Wendisch</name>
</region>
<name sortKey="Agostini, Federica" sort="Agostini, Federica" uniqKey="Agostini F" first="Federica" last="Agostini">Federica Agostini</name>
<name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name>
<name sortKey="Freiwald, Anja" sort="Freiwald, Anja" uniqKey="Freiwald A" first="Anja" last="Freiwald">Anja Freiwald</name>
<name sortKey="Hippenstiel, Stefan" sort="Hippenstiel, Stefan" uniqKey="Hippenstiel S" first="Stefan" last="Hippenstiel">Stefan Hippenstiel</name>
<name sortKey="Hocke, Andreas" sort="Hocke, Andreas" uniqKey="Hocke A" first="Andreas" last="Hocke">Andreas Hocke</name>
<name sortKey="Kilian, Christiane" sort="Kilian, Christiane" uniqKey="Kilian C" first="Christiane" last="Kilian">Christiane Kilian</name>
<name sortKey="Kuebler, Wolfgang M" sort="Kuebler, Wolfgang M" uniqKey="Kuebler W" first="Wolfgang M" last="Kuebler">Wolfgang M. Kuebler</name>
<name sortKey="Kurth, Florian" sort="Kurth, Florian" uniqKey="Kurth F" first="Florian" last="Kurth">Florian Kurth</name>
<name sortKey="Ludwig, Daniela" sort="Ludwig, Daniela" uniqKey="Ludwig D" first="Daniela" last="Ludwig">Daniela Ludwig</name>
<name sortKey="Michalick, Laura" sort="Michalick, Laura" uniqKey="Michalick L" first="Laura" last="Michalick">Laura Michalick</name>
<name sortKey="Mulleder, Michael" sort="Mulleder, Michael" uniqKey="Mulleder M" first="Michael" last="Mülleder">Michael Mülleder</name>
<name sortKey="Pfeiffer, Moritz" sort="Pfeiffer, Moritz" uniqKey="Pfeiffer M" first="Moritz" last="Pfeiffer">Moritz Pfeiffer</name>
<name sortKey="Ralser, Markus" sort="Ralser, Markus" uniqKey="Ralser M" first="Markus" last="Ralser">Markus Ralser</name>
<name sortKey="Sander, Leif Erik" sort="Sander, Leif Erik" uniqKey="Sander L" first="Leif Erik" last="Sander">Leif Erik Sander</name>
<name sortKey="Suttorp, Norbert" sort="Suttorp, Norbert" uniqKey="Suttorp N" first="Norbert" last="Suttorp">Norbert Suttorp</name>
<name sortKey="Textoris Taube, Kathrin" sort="Textoris Taube, Kathrin" uniqKey="Textoris Taube K" first="Kathrin" last="Textoris-Taube">Kathrin Textoris-Taube</name>
<name sortKey="Thibeault, Charlotte" sort="Thibeault, Charlotte" uniqKey="Thibeault C" first="Charlotte" last="Thibeault">Charlotte Thibeault</name>
<name sortKey="Von Kalle, Christof" sort="Von Kalle, Christof" uniqKey="Von Kalle C" first="Christof" last="Von Kalle">Christof Von Kalle</name>
<name sortKey="Witzenrath, Martin" sort="Witzenrath, Martin" uniqKey="Witzenrath M" first="Martin" last="Witzenrath">Martin Witzenrath</name>
</country>
<country name="Suède"><noRegion><name sortKey="Zelezniak, Aleksej" sort="Zelezniak, Aleksej" uniqKey="Zelezniak A" first="Aleksej" last="Zelezniak">Aleksej Zelezniak</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/CovidFranceV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000319 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000319 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    CovidFranceV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:32619549
   |texte=   Ultra-High-Throughput Clinical Proteomics Reveals Classifiers of COVID-19 Infection.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:32619549" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a CovidFranceV1

This area was generated with Dilib version V0.6.37.
Data generation: Tue Oct 6 23:31:36 2020. Site generation: Fri Feb 12 22:48:37 2021

	Serveur d'exploration sur la COVID en France
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur la COVID en France

Ultra-High-Throughput Clinical Proteomics Reveals Classifiers of COVID-19 Infection.

Ultra-High-Throughput Clinical Proteomics Reveals Classifiers of COVID-19 Infection.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki